Journal: Science Advances

Article Title: Quantizing single-molecule surface-enhanced Raman scattering with DNA origami metamolecules

doi: 10.1126/sciadv.aau4506

Figure Lengend Snippet: ( A ) Schematic illustration of the fabrication procedure. L-AuNPs (80 nm) and dyes could be immobilized site-specifically on a rhombus-shaped super-origami through DNA hybridizations. ( B ) FDTD calculations for an 80-nm L-AuNP tetramer cluster. A hot spot is present in the green box. ( C to E ) Correlative SEM characterization (C), DFM characterization (D), and Raman mapping (E) of an 80-nm L-AuNP tetramer cluster. Six ROX (carboxy-X-rhodamine) molecules were placed in the hot spot shown in (B). a.u., arbitrary units.

Article Snippet: FDTD simulations of tetrameric metamolecules (particle diameter, 80 nm; gap size, 2 nm) were carried out using Lumerical FDTD solutions (Lumerical Solutions, Canada).

Techniques:

Journal: Science Advances

Article Title: Quantizing single-molecule surface-enhanced Raman scattering with DNA origami metamolecules

doi: 10.1126/sciadv.aau4506

Figure Lengend Snippet: ( A ) Schematic of the tetrameric metamolecule that is incorporated with Raman dye. ( B ) Real-color photograph and the corresponding SEM images of the two individual tetramers (i and ii). Scale bars, 1 μm. ( C ) High-magnification SEM images reveal the difference between two tetramers. Scale bars, 100 nm. ( D ) FDTD calculations for two tetramer clusters. Scale bars, 50 nm. ( E ) Nonpolarized experimentally scattering spectra of the two individual tetramers. ( F ) Raman spectra of individual tetramers with intercalated SYBR Green I molecules (spectra i and ii) and the highly concentrated bulk solution (black curve) of SYBR Green I. All measurements were performed with a 633-nm excitation laser (10-s exposure).

Article Snippet: FDTD simulations of tetrameric metamolecules (particle diameter, 80 nm; gap size, 2 nm) were carried out using Lumerical FDTD solutions (Lumerical Solutions, Canada).

Techniques: SYBR Green Assay